Screening and Identification of An Organic-solvent-stable Protease-producing Strain

doi:10.7506/spkx1002-6630-200907045

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 197-201.doi: 10.7506/spkx1002-6630-200907045

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Screening and Identification of An Organic-solvent-stable Protease-producing Strain

FANG Yao-wei1 LIU Shu1 XU Wei-feng2 WANG Shu-jun1 LU Ming-sheng1 CHEN Li1

(1.Huaihai Institute of Technology, Jiangsu Key Laboratory of Marine Biotechnology, Lianyungang 222005, China
2.Jiangsu Agro-product Quality Test Center, Nanjing 210036, China )

Received:2008-07-10 Revised:2008-11-28 Online:2009-04-01 Published:2010-12-29
Contact: FANG Yao-wei1 E-mail:nnfyw◎yahoo.com.cn

Abstract

Abstract:

In this study, 56 organic-solvent-tolerant strains were isolated from oil-pollution water by using MLB medium with 10% benzene (V/V) addition. Among them, the high-yield strain DS1 was finally obtained through the first screening with SMA plate and the second screening with shake flask fermentation. The strain DS1 was identified as Bacillus aquaemaris by its morphological, physiological, biochemical characteristics and 16S rDNA sequence homology. The verifying test showed that the strain DS1 can tolerant organic solvents at different levels, and the organic-solvent-tolerant protease excreted by strain DS1 also has a nice tolerance to organic solvents. This protease is stable in 25% organic solvents with logPow equal to or more than 1.8, and may keep above 70% activity after 14-d cultivation at 30 ℃ and 140 r/min. It was expected that both of the strain DS1 and its protease can be used as catalysts for reactions in organic phase, and have huge potential in industrial application.

Key words: organic-solvent-tolerant microorganism, organic-solvent-stable protease, screening, identification

CLC Number:

TS201.25

FANG Yao-wei1 LIU Shu1 XU Wei-feng2 WANG Shu-jun1 LU Ming-sheng1 CHEN Li1. Screening and Identification of An Organic-solvent-stable Protease-producing Strain[J]. FOOD SCIENCE, 2009, 30(7): 197-201.

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Screening and Identification of An Organic-solvent-stable Protease-producing Strain

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