FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (7): 299-303.doi: 10.7506/spkx1002-6630-200907069

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Sequence Analysis of 16S rRNA and Its Application in Classification and Identification of Lactic Acid Bacteria

ZHAO Yu-juan,NIU Chun-hua,ZHANG Xue,LI Da,ZHANG Jian,YANG Zhen-nai   

  1. Center of Agro-Food Technology, Jilin Academy of Agricultural Sciences, Changchun 130124, China
  • Received:2008-05-29 Revised:2008-06-12 Online:2009-04-01 Published:2010-12-29
  • Contact: YANG Zhen-nai E-mail:zyang@cjaas.com

Abstract:

The characteristics of 16S rRNA and the fundamental methodology of its sequence analysis were reviewed in this paper. Several molecular biological methods related to 16S rRNA sequence analysis were detailed to classify and identify lactic acid bacteria such as direct sequencing, oligonucleotide probes, the polymorphism of restriction fragment length, temperature gradient gel electrophoresis. The recent progresses in the application of these methods were also introduced in detail.

Key words: lactic acid bacteria, 16S rRNA, classification, identification

CLC Number: