Abstract:

The effects of four factors such as temperature, sodium hydroxide concentration, time and ratio of material to sodium hydroxide solution on the yield of polysaccharide from Evodia rutaecarpa (Juss) Benth were investigated, and these factors were optimized through orthogonal test. The results showed that ratio of material to sodium hydroxide solution is the most distinct factors affecting the polysaccharide yield, and the optimum ratio of material to sodium hydroxide solution, extraction time, temperature and sodium hydroxide concentration are 1:20, 3 h, 80 ℃ and 0.6 mol/L, respectively. Under the above conditions, the yield of polysaccharide is 12.58%. Two protein removal methods of Sevag and TCA were compared. The results indicated that the Sevag method has the advantage of the lower loss, but consumes more time and solvent, while the TCA method has a higher efficiency to remove protein and may save a lot of time and solvent. Altogether, the TCA method is better for removing protein from polysaccharide.

Key words: Evodia rutaecarpa (Juss) Benth, polysaccharide, extraction, protein removal