FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (8): 155-157.doi: 10.7506/spkx1002-6630-200908031

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Analysis of Limonene in Navel Orange Juice by HS-SPME

PAN Jian WANG Hai-xiang* XIE Hui-ming YANG Yi ZENG Qing-mei   

  1. (Engineering Research Center of Bio-Process, Ministry of Education, Hefei University of Technology, Hefei 230009 China)
  • Received:2008-05-09 Revised:2008-06-22 Online:2009-04-15 Published:2010-12-29
  • Contact: WANG Hai-xiang* E-mail:wangsky111@yeah.net

Abstract:

A method based on headspace solid - phase microextraction (HS-SPME) was developed to analyze limonene in navel orange juice, and some key factors such as SPME fiber, temperature, time and NaCl concentration were investigated. The optimum conditions of SPME-GC are extraction at 50 ℃ for 30 min with addition of 0.3 g/ml NaCl in 100μm of PDMS fiber. The study on recovery of standard addition, linear range and repeatability showed that this method has high sensitivity, precision and good liner relationship, and can be used for detection of limonene in orange juice.

Key words: HS-SPME, GC-MS, limonene

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