FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (12): 61-65.doi: 10.7506/spkx1002-6630-200912008

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Ultrasonic-assisted Enzymatic Hydrolysis of Corn Protein

LIU Zhen-chun,DENG Zi-yu,LI Hui,DUAN Xu   

  1. (College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China)
  • Received:2008-09-22 Revised:2008-12-29 Online:2009-06-15 Published:2010-12-29
  • Contact: LIU Zhen-chun E-mail:liuzhenchun63@163.com

Abstract:

The present study aimed to develop a combination method of ultrasonic pre-treatment and alkaline proteasecatalyzed hydrolysis for corn protein. The ultrasonic-assisted enzymatic hydrolysis was optimized through orthogonal test design based on single factor test. When ultrasonic treatment duration, mass fractions of substrate and enzyme, and pH value and temperature for hydrolysis were 4 min, 2% and 5%, and 10.5 and 55 ℃, respectively, the maximum hydrolysis degree was achieved, reaching 61.47%. Moreover, molecular weights of products obtained at different hydrolysis time were determined by SDS-PAGE.

Key words: corn protein, hydrolysis degree, ultrasonic

CLC Number: