Abstract:

An immunocapture-universal primer polymerase chain reaction (PCR) (IC-UPPCR) method was developed by combining the floride ion-selective electrode method with classical PCR assay for detection of Salmonella sp. in food and its sensitivity and specificity were investigated. It was feasible to establish a universal primer PCR technology was by designing specific primers from conserved regions of bacterial 16S ribosomal RNA genes. The sensitivity of the IC-UPPCR to detect Salmonella sp. was 2 × 102 CFU/ml and the specificity was 100% without any false negative or positive cases. The results demonstrate that the IC-UPPCR is a simple, rapid, highly sensitive and specific method for the detection of Salmonella sp. in the foods and suitable for detection of Salmonella sp. in a large number of food samples.

Key words: immunocapture, universal primer, PCR, Salmonella sp., foods