Abstract:

The isolation of large quantities of good-quality RNA from fruit tissues rich in polysaccharide or polyphenol has been a long-standing problem for researchers. In this study, a self-prepared TRIZOL reagent was used to extract total RNA from tomato (rich in polysaccharide). Also, an improved PVP method was applied to extract total RNA from grape (rich in polyphenol). Compared to guanidinium thiocyanate method, the above methods resulted in higher purity and yield of total RNA from tomato and grape, respectively.

Key words: fruit tissue, total RNA, TRIZOL, PVP