FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (13 ): 223-226.doi: 10.7506/spkx1002-6630-200913051

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Preparation and Identification of Monoclonal Antibody against Malachite Green

LI Zhao-hui，JIANG Pan-pan，HE Ji-guo*   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083,China
  • Received:2009-04-08 Online:2009-07-01 Published:2010-12-29
  • Contact: HE Ji-guo* E-mail:hejiguo0870@sina.com

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Abstract:

Balb/c Mice were immunized with the malachite green (MG) coupled with bovine serum albumin, and a strain of hybridoma cell named 5G6, which can stably secret monoclonal antibody (McAb), were obtained by cell hybridoma technology. The subclass of the McAb was IgG1, and the light chain was κ type. The ascites titer determined by indirect ELISA was 1.28 ×105, while the indirectly competitive ELISA showed that its IC50 to malachite green was 1.36 ng/ml. Except crystal violet (CV), the McAb had no cross-reaction with leucomalachite green (LMG).

Key words: malachite green (MG), monoclonal antibody (McAb), enzyme-linked immune sorbent assay

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