Abstract:

Objective: To study the effects of ovum oil of Rana chensinensis on antioxidant capacity in mice exposed to cold stress to lay a foundation for rational development and utilization of the oil. Methods: Forty male Kunming mice were randomly divided into four groups: control group, cold stress group, 5-time dose group of the oil and 25-time dose group of the oil, with 10 mice in each group. The two dose groups were subjected to a 28-day gastric perfusion, and the control group and the cold stress group were treated with the same amount of saline. A 30-min cold stress treatment to others except the control group mice began in －10 ℃ refrigerator 30 min after the gastric perfusion since the 15th day. The superoxide dismutase (SOD) activity and the MDA content in blood and brain tissues of newly sacrificed mice were measured. Results: The oil increased the activity of SOD (p < 0.01) in mice blood and brain tissues, and reduced the content of MDA (p < 0.01). Conclusion: The ovum oil of Rana chensinensis can enhance the antioxidant capacity in mice exposed to cold stress.

Key words: ovum oil of Rana chensinensis, cold stress, antioxidant capicity