FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (14 ): 285-287.doi: 10.7506/spkx1002-6630-200914063

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Liquid Chromatography/Triple Quadrupole Tandem Mass Spectrometric Determination of Chloramphenicol Residue in Honey

TIAN Wen-li,PENG Wen-jun*,HAN Sheng-ming,GAO Ling-yu,GUO Zhan-bao   

  1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, C
  • Received:2009-03-16 Online:2009-07-15 Published:2010-12-29
  • Contact: PENG Wen-jun*, E-mail:pengwenjun@vip.sina.com

Abstract:

A LC-MS/MS method was developed for the detection of chloramphenicol residue in honey. Samples were extracted using ethyl acetate, and the extracts were separated on a ZORBAX XDB-C18 column (2.1 mm×150 mm, 5 μm) with a mobile phase composed of methanol and water (40:60, V/V). The analysis was carried out by MS/MS in an electrospray negative ion multiple reaction monitoring (MRM) mode. The limits of detection and quantification were 0.1 ng/ml and 0.1 g/kg, respectively. The linear range was between 0.1 ng/ml and 10.0 ng/ml. The average spike recovery was 88.2%, with an average RSD of 3.26%.

Key words: LC-MS/MS, chloramphenicol, honey, residue

CLC Number: