Identification of A Novel Bacillus Strain Capable of Producing Neutral β-Glucosidase and Characterization of Its Enzymatic Properties

doi:10.7506/spkx1002-6630-200915036

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (15): 160-163.doi: 10.7506/spkx1002-6630-200915036

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Identification of A Novel Bacillus Strain Capable of Producing Neutral β-Glucosidase and Characterization of Its Enzymatic Properties

CHEN Hong-man，ZHAO Lu，YANG Jia-ying

Shenyang Agricultural University, Shenyang 110161, China

Received:2008-10-30 Revised:2009-06-12 Online:2009-08-01 Published:2010-12-29
Contact: CHEN Hong-man， E-mail:hongmanc@hotmail.com

Abstract

Abstract:

A novel β-glucosidase-producing Bacillus strain ZL-110 was isolated from basic soil. The taxonomic position of this bacterium was investigated on the basis of 16S rRNA gene sequencing. Comparative computer database analyses revealed that the bacterium is a Bacillus subtilis strain. Based on the alignment of 16S rRNA sequences, we concluded that the novel B. subtilis strain ZL-110 is most closely related to the B. subtilis reference strain M01. SDS-PAGE analysis indicated that this β- glucosidase has a MW of 43 kD. When salicin was used as a substrate, the optimum reaction conditions for this enzyme were pH 7.0 and 50 ℃. In addition, this neutral enzyme demonstrated excellent thermostability.

Key words: neutral enzyme, β-glucosidase, 16S rDNA, enzymatic properties

CLC Number:

TS201.3

CHEN Hong-man，ZHAO Lu，YANG Jia-ying. Identification of A Novel Bacillus Strain Capable of Producing Neutral β-Glucosidase and Characterization of Its Enzymatic Properties[J]. FOOD SCIENCE, 2009, 30(15): 160-163.

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Identification of A Novel Bacillus Strain Capable of Producing Neutral β-Glucosidase and Characterization of Its Enzymatic Properties

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