Mixture Design Optimization of Cellulase Complex Formulation

doi:10.7506/spkx1002-6630-200915038

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (15): 169-171.doi: 10.7506/spkx1002-6630-200915038

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Mixture Design Optimization of Cellulase Complex Formulation

LIN Zeng-xiang1，ZHANG Hong-man2，YAN Li-shi1，CHEN Jing-wen1，HU Ling1，YU Wen-tao1，HUANG He1,*

1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Pharmacy and Life Science, Nanjing University of
Technology, Nanjing 210009, China；2. Department of Applied Chemistry, Nanjing University of Technology, Nanjing 210009, China

Received:2008-09-25 Revised:2009-06-16 Online:2009-08-01 Published:2010-12-29
Contact: HUANG He1,* E-mail:biotech@njut.edu.cn

Abstract

Abstract:

Three different commercial cellulase preparations were used to prepare complex enzyme to increase the hydrolysis efficiency of cellulase. The formulation of cellulose complex was optimized by mixture design to attain the maximum concentration of reduced sugar in hydrolysate solution and a mathematical model describing the relationship of concentration of reduced sugar with volumes of mixed cellulases was developed. A mixture of Zesheng cellulase and Genencor cellulase at a volume ratio of 1:1 resulted in the maximum hydrolysis efficiency higher than that of individual cellulase by 39.5%. Mixture design is available for the optimization of cellulase complex formulation.

Key words: mixture design, cellulase, complex formulation, cellulose

CLC Number:

Q396

LIN Zeng-xiang1，ZHANG Hong-man2，YAN Li-shi1，CHEN Jing-wen1，HU Ling1，YU Wen-tao1，HUANG He1,*. Mixture Design Optimization of Cellulase Complex Formulation[J]. FOOD SCIENCE, 2009, 30(15): 169-171.

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Mixture Design Optimization of Cellulase Complex Formulation

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