FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (16): 73-77.doi: 10.7506/spkx1002-6630-200916010

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Preparation and Sulfation of Polysaccharides from Pumpkin

KONG Qian,ZHOU Ting,WU Gai-lan,HUANG Jing-rong,LI Quan-hong*   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Received:2008-08-27 Revised:2009-06-16 Online:2009-08-15 Published:2010-12-29
  • Contact: LI Quan-hong E-mail:liquanhong66@163.com

Abstract:

Crude water-soluble polysaccharides were separated from pumpkin by hot water extraction and gradient ethanol precipitation, and purified/fractionated by DEAE-Sepharose Fast Flow anion-exchange chromatography and a acidic polysaccharide PL-2 was obtained. Sepharose CL-6B gel filtration chromatography and HPLC analysis demonstrated that the component of PL-2 was homogenous and its molecular weight was more than 2000 kD. The polysaccharide was chemically modified by chlorosulfuric acid-pyridine method, sulfur trioxide-pyridine method and sulfamic acid, respectively. The identification of BaCl2-gelatin turbidimetry and infrared spectra revealed that sulfur trioxide-pyridine method displayed the best sulfation effect.

Key words: pumpkin polysaccharide, purification, sulfation

CLC Number: