FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (16 ): 119-122.doi: 10.7506/spkx1002-6630-200916021

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Use of Freezing Wall-breaking Method for the Extraction of Barley Green from Buckwheat Seedlings

WANG Wei-jian1,GUO Li-quan2,LENG Jin-song1   

  1. 1. Department of Food Engineering, Jilin Business and Technology College, Changchun 130062, China;
    2. Department of Biology Engineering, Jilin Business and Technology College, Changchun 130062, China
  • Received:2009-06-02 Online:2009-08-15 Published:2010-12-29
  • Contact: WANG Wei-jian E-mail:wwjflora@yahoo.com.cn

Abstract:

A liquid product of barley green was prepared from buckwheat seedlings by the steps of freezing wall breaking, extraction with 1/150 mmol/L phosphate buffer containing 0.01% sodium erythorbate by grinding, vacuum concentration and instantaneous ultra-high temperature sterilization. The freezing wall breaking extraction of buckwheat seedlings was optimized by orthogonal array design. Under optimized conditions, the contents of protein, chlorophyll and rutin and the SOD activity of barley green extract were 0.26%, 9.37 mg/100 ml, 1.03 mg/100 ml, and 242 U/100 ml, respectively. Other indexes of the product obtained were in accordance with the national standards for plant health products.

Key words: buckwheat, barley green, freeze wall breaking, rutin

CLC Number: