Abstract:

Total flavonoids in Flos Chrysanthemi were extracted by wet superfine comminution technology. Single-factor method was adopted to investigate the effects of gap between colloid mill teeth, ethanol concentration, extraction temperature and extraction time on the extraction yield of total flavonoids was investigated. Subsequently, an orthogonal array design involving 9 experiments of the four factors at three levels was employed to attain the highest extraction yield of total flavonoids. The optimum extraction conditions were determined as follows: gap between colloid mill teeth 20 μm, ethanol concentration 75%, temperature 70 ℃ for an extraction duration of 20 min. Under these conditions, the extraction yield of total flavonoids was 3.45%. A high extraction yield can be achieved in a short period of time using wet superfine comminution. In general, this technology is a promising approach for the extraction of total flavonoids from Flos Chrysanthemi.

Key words: superfine comminution, total flavonoids, Flos Chrysanthemi, extraction technology