Separation, Purification and Amino Acid Composition Analysis of Zinc-rich Water-soluble Protein ZnP6B-G75-WPⅡfrom Water Shield Leaves

doi:10.7506/spkx1002-6630-200917019

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (17 ): 88-90.doi: 10.7506/spkx1002-6630-200917019

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Separation, Purification and Amino Acid Composition Analysis of Zinc-rich Water-soluble Protein ZnP6B-G75-WPⅡfrom Water Shield Leaves

ZHOU Yi-feng1，TANG Qiao-yu2，ZHOU Da-zhai2，WU Yong-yao3

1. School of Biological Science and Technology, Hubei University for Nationalities, Enshi 445000, China；
2. Key Laboratory for Protection and Utilization of Biological Resources of Hubei Province, Enshi 445000, China ；
3. Laboratory of Biochemical and Fermentation Engineering, Hunan Agricultural University, Changsha 410128, China

Received:2009-06-17 Online:2009-09-01 Published:2014-04-14

Abstract

Abstract:

A zinc rich water-soluble protein fraction named ZnP6B-G75-WPⅡwas separated from a zinc-rich water-soluble protein WP Ⅱ extracted from water shield leaves by Sephadex G-75 column chromatography and purified by Sepharose 6B column chromatography. The purity and the amino acid composition of ZnP6B-G75-WPⅡwere analyzed by SDS-PAEG and RT-HPLC, respectively. SDS analysis indicated that the molecule of ZnP6B- G75-WPⅡ was composed by a single subunit. The relative content of zinc was 8.44 mg/kg. ZnP6B- G75-WP Ⅱ was composed of 12 amino acids including glycin of high content and others such as histidine, cysteine, selenium-cysteine, arginine etc. which are related with zinc combination and selenium-cysteine was found to be contained in the protein.

Key words: water shield, zinc, water-soluble protein, separation and purification

CLC Number:

TS201.7

ZHOU Yi-feng1，TANG Qiao-yu2，ZHOU Da-zhai2，WU Yong-yao3. Separation, Purification and Amino Acid Composition Analysis of Zinc-rich Water-soluble Protein ZnP6B-G75-WPⅡfrom Water Shield Leaves[J]. FOOD SCIENCE, 2009, 30(17 ): 88-90.

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Separation, Purification and Amino Acid Composition Analysis of Zinc-rich Water-soluble Protein ZnP6B-G75-WPⅡfrom Water Shield Leaves

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