FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (17): 181-184.doi: 10.7506/spkx1002-6630-200917042

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Structural Characterization of Alcalase Hydrolysates of Soybean 7S Globulin

DUAN Chun-hong,YAO Xiao-lin,ZHU Xin-rong,SUN Wan,PAN Si-yi*   

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2009-05-25 Online:2009-09-01 Published:2014-04-14
  • Contact: PAN Si-yi* E-mail:pansiyi@mail.huau.edu.cn

Abstract:

Soybean 7S and 11S globulins, two major soybean protein components, play an important role in maintaining the phisico-chemical properties of soybean proteins. The structural properties of both soybean 7S globulin and its hydrolysates were investigated by SDS-PAGE, SEM and FTIR infrared spectroscopy, meanwhile their hydrophobicity and sulfhydryl content were measured. That alcalase-catalyzed hydrolysis led to marked conformational changes was observed in soybean 7S globulin and the degree of change was dependant on the degree of hydrolysis.

Key words: 7S globulin, alcalase, hydrolysis, structural properties

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