Purification and Free Radical Scavenging of Fruit-body Polysaccharide from Pleurotus nebrodensis

doi:10.7506/spkx1002-6630-200919023

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (19): 109-113.doi: 10.7506/spkx1002-6630-200919023

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Purification and Free Radical Scavenging of Fruit-body Polysaccharide from Pleurotus nebrodensis

MA Shu-feng1，CHEN Li-mei2，XU Hua-neng1，LIU Chang-jiang3

1. School of Food Science and Technology, Jiangnan University, Wuxi 214122, China；
2. Department of Food Science, College of Agricucture, Liaocheng University, Liaocheng 252059, China；
3. College of Food, Shenyang Agricultural University, Shenyang 110161, China

Received:2009-06-18 Revised:2009-08-11 Online:2009-10-01 Published:2010-12-29
Contact: MA Shu-feng1 E-mail:msf971516@126.com

Abstract

Abstract:

A water-soluble polysaccharide was isolated and purified from fruit-body of Pleurotus nebrodensis through sequential ethanol precipitation by Savag method, DEAE-cellulose 52 and Sephadex G-200 column chromatography to remove proteins and small molecules. This polysaccharide was identified to be PNFP-IIa through UV, agarose gel electrophoresis and Sephadex G-200 column chromatography. Moreover, PNFPII-a revealed high scavenging capability against hydroxyl and superoxide anion free radicals through chemiluminescent assay.

Key words: Pleurotus nebrodensis, fruit-body polysaccharide, purification, PNFP II-a, free radical scavenging

CLC Number:

TS201.1

MA Shu-feng1，CHEN Li-mei2，XU Hua-neng1，LIU Chang-jiang3. Purification and Free Radical Scavenging of Fruit-body Polysaccharide from Pleurotus nebrodensis[J]. FOOD SCIENCE, 2009, 30(19): 109-113.

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Purification and Free Radical Scavenging of Fruit-body Polysaccharide from Pleurotus nebrodensis

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