FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (19 ): 203-206.doi: 10.7506/spkx1002-6630-200919046

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Immobilized Neutrase-catalyzed Hydrolysis of Walnut Protein and Properties of Its Hydrolysates

XUE Yang,HUANG Li,QI Bin, WANG Li-mei*   

  1. Fermentation Engineering Technology Center, Changshu Institute of Technology, Changshu 215500, China
  • Received:2009-06-15 Online:2009-10-01 Published:2010-12-29
  • Contact: XUE Yang

Abstract:

Hydrolysis procedure of walnut protein by calcium alginate immobilized neutrase 0.8 L with retention of original enzyme activity of 57.41% was studied and the hydrolysates were analyzed for antioxidant activity and amino acid composition.  The optimal pH and temperature for immobilized neutrase to hydrolyze walnut protein were 6.0 and 50 ℃, respectively. The highest degree of hydrolysis was 18.37% through 5 h reaction at pH 6.0 and 50 ℃ with an enzyme dose of 3.5 g/L. The hydrolysates of walnut protein exhibited obvious reducing power and provided effective protection against oxidation of linoleic acid.

Key words: immobilization, walnut, degree of hydrolysis, antioxidation, neutrase

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