FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (2): 46-50.doi: 10.7506/spkx1002-6630-201002011

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Optimization of Extraction Technology of Polysaccharides from Petasites tricholobus Roots by Response Surface Analysis

WANG Hong,DENG Ze-yuan*,LIU Rong,FAN Ya-wei,LI Jing   

  1. State Key Laboratory of Food Science and Technology, Institute of Advanced Studies, Nanchang University,
    Nanchang 330047, China
  • Received:2009-03-25 Revised:2009-08-10 Online:2010-01-15 Published:2010-12-29
  • Contact: DENG Ze-yuan*, E-mail:dengzy28@yahoo.com.cn

Abstract:

Crude polysaccharides were prepared from the residue of Petasites tricholobus roots after flavonoids extraction. According to single-factor experiments, three levels of the factors including extraction temperature, extraction time and liquid/solid ratio were selected for the central composite design to establish a quadric regression equation for predicting the yield of polysaccharides. By response surface analysis, the optimum extraction conditions were obtained as follows: extraction temperature 70 ℃, extraction time 2.3 h and liquid/solid ratio 45:1, which led to estimated and observed values of maximal yield of polysaccharides of 35.04% and 34.74%, respectively.

Key words: Petasites tricholobus roots, polysaccharides, extraction technology, response surface methodology

CLC Number: