FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (2 ): 98-101.doi: 10.7506/spkx1002-6630-201002023

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Extraction of Protein and Speciation Analysis of Zinc in Zingiber striolatum

LUO Xing-wu1,ZHOU Da-zhai2,ZHU Yu-chang2,WAN Li-xiu1   

  1. (1. School of Biological Science and Technology, Hubei Institute for Nationalities, Enshi 445000, China;
    2. Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Biological Technology Institute,
    Hubei University for Nationalities, Enshi 445000, China)
  • Received:2009-06-23 Online:2010-01-15 Published:2010-12-29
  • Contact: LUO Xing-wu1, E-mail:lxwmss@163.com

Abstract:

The optimal extraction process of protein from Zingiber striolatum determined using single factor method coupled with orthogonal array design was that Zingiber striolatum powder was extracted with PBS (pH 9.0) at 60 ℃ for 60 minutes with a solid-to-solvent ratio of 1:40 (g/mL). Additionally, speciation analysis of zinc in Zingiber striolatum was carried out. Results revealed that organic zinc constituted a major fraction in Zingiber striolatum, which accounted for above 80% of total zinc and comprised above 65% protein-binding zinc.

Key words: Zingiber striolatum, protein, optimization of extraction, zinc, speciation

CLC Number: