Antioxidant Activity of Water-soluble Matrix from Abalone Shell

doi:10.7506/spkx1002-6630-201009027

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 113-116.doi: 10.7506/spkx1002-6630-201009027

• Basic Research • Previous Articles Next Articles

Antioxidant Activity of Water-soluble Matrix from Abalone Shell

LIANG Hong-bao1，GUO Wei1，DONG Sheng-xiong1，WU Jiu-lin1，PENG Zhen-fei1，LIU Zhi-bin1，ZHANG Qi-qing1,2,*

1. Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China ；
2. Institute of Biomedical Engineering, Chinese Academy of Medical Sciences, Tianjin 300192, China)

Received:2009-09-11 Revised:2010-01-04 Online:2010-05-01 Published:2010-12-29
Contact: ZHANG Qi-qing1,2,* E-mail:zhangqiq@126.com

Abstract

Abstract:

The water-soluble matrix from abalone shell (WSMA) was purified by ion exchange chromatography (IEC) in DEAESephadex A-25 column. The antioxidant activity of WSMA was evaluated by scavenging capabilities of DPPH (1,1-diphenyl- 2-picrylhydrazy), hydroxyl (·OH) and superoxide anion (O2·) free radicals, and reducing powder of ferric ions. Compared with water-soluble matrix from pearl (WSMP) and vitamin C, WSMA revealed the similar reducing power and scavenging capability to O2· with WSMP, however, both WSMA and WSMP exhibited a weak inhibition effect on DPPH· and ·OH. The fraction from the second peak (AⅡ) separated by IEC was the major bioactive component. The scavenging capability of 1.0 mg/mL AⅡ to superoxide anion free radicals was 80%, which was equivalent to 0.1 mg/mL vitamin C.

Key words: abalone shell, water-soluble matrix, antioxidant activity, ion exchange chromatography

CLC Number:

TS254.4

LIANG Hong-bao1，GUO Wei1，DONG Sheng-xiong1，WU Jiu-lin1，PENG Zhen-fei1，LIU Zhi-bin1，ZHANG Qi-qing1,2,*. Antioxidant Activity of Water-soluble Matrix from Abalone Shell[J]. FOOD SCIENCE, 2010, 31(9): 113-116.

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Antioxidant Activity of Water-soluble Matrix from Abalone Shell

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