Screening, Identification and Breeding of Conjugated Linoleic Acid-producing Lactobacillus plantarum by Mutation

doi:10.7506/spkx1002-6630-201009032

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 135-139.doi: 10.7506/spkx1002-6630-201009032

• Bioengineering • Previous Articles Next Articles

Screening, Identification and Breeding of Conjugated Linoleic Acid-producing Lactobacillus plantarum by Mutation

LIU Pei，ZHOU Qian，SHEN Sheng-rong，RUAN Hui，MA Liu-liu，HE Guo-qing*

School of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310029, China

Received:2009-08-26 Revised:2009-12-18 Online:2010-05-01 Published:2010-12-29

Abstract

Abstract:

A lactic acid bacterial strain, lp15, having the ability to synthesize conjugated linoleic acid (CLA) was isolated from pickle juice samples. The strain lp15 was identified as Lactobacillus plantarum based on full-length 16S rRNA sequence analysis and API system. This strain was treated by ultraviolet and DES to obtain several mutant strains, which exhibited 29.3%－52.2% enhancement for CLA production. The strain lp15-2-1 exhibited the highest CLA productivity, which was up to 30.13 μg/mL after 48 h culture in the MRS medium added with LA at 0.2 mg/mL level. According to gas chromatographic analysis, cis9, trans11-CLA was the predominant compound in the fermentation product, with a relative content of up to 76.5%. trans10, cis12-CLA approximately accounted for 23.5% of the fermentation product.

Key words: conjugated linoleic acid (CLA), Lactobacillus plantarum, identification, mutation, gas chromatography (GC)

CLC Number:

TS218

LIU Pei，ZHOU Qian，SHEN Sheng-rong，RUAN Hui，MA Liu-liu，HE Guo-qing*. Screening, Identification and Breeding of Conjugated Linoleic Acid-producing Lactobacillus plantarum by Mutation[J]. FOOD SCIENCE, 2010, 31(9): 135-139.

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Screening, Identification and Breeding of Conjugated Linoleic Acid-producing Lactobacillus plantarum by Mutation

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