FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 150-155.doi: 10.7506/spkx1002-6630-201009035

• Bioengineering • Previous Articles     Next Articles

Screening and Identification of Antagonistic Bacteria against Post-harvest Diseases of Jujube Fruits

GENG Hai-feng1,ZHANG Li-zhen2,*,NIU Wei3   

  1. 1. College of Biological Engineering, Shanxi University, Taiyuan 030006, China;2. College of Life Science and Technology, Shanxi
    University, Taiyuan 030006, China;3. Shanxi Academy of Agricultural Science, Taiyuan 030006, China
  • Received:2009-09-03 Online:2010-05-01 Published:2010-12-29

Abstract:

Eleven strains of antagonistic bacteria were isolated from soil samples and exhibited an excellent antagonistic effect on Alternaria alternate, Fusarium decemcellulare Brick and Monilia fructicola. Results showed strain B26 had the strongest inhibition effect on three selected fungal pathogens. The average radius of inhibition zone was 15.8 mm in plate inhibition test and inhibition rate against Alternaria alternate was 78.8%. Similarly, strain B26 also exhibited inhibition effect on Fusarium decemcellulare Brick and Monilia fructicola with average radius of 14.3 mm and 12.8 mm, and inhibition rate of 71.3 % and 63.8%, respectively. Strain B26 was primarily identified as Bacillus subtilis through the characterization of morphological features, cultural characteristic observation PDA, physiological and biochemical measurements and 16S rDNA sequence analysis of PCR products.

Key words: Jujube fruit, post-harvest diseases, antagonistic bacteria, screening, identification

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