Abstract:

Objective: To explore the purification method and hypolipidemic effect of polysaccharides from fruit body of Pholiota adipose polysaccharides. Methods: The crude polysaccharides were purified after removing protein by neutral protease and Sevag method. Experimental mice were divided into five groups including NS control group, the model group with high fat diet, the treatment groups fed with purified polysaccharides at the low-dosage of 100 mg/(kg bw·d), the medium-dosage of 200 mg/(kg bw·d) and the high-dosage of 400 mg/(kg bw·d). Due to the high fat diet, the model of hyperlipidemia was successfully established. After five weeks, the contents of serum TC, TG, high-density cholesterol (HDL-C), low-density cholesterol (LDL-C) were determined. Results: Purified polysaccharides from Pholiota adipose at three dosages exhibited the significant decrease in TC (high-dosage group and low-dosage group P＜0.05), TG (medium-dosage group P＜0.05, high-dosage group and low-dosage group P＜0.01), LDL-C (medium-dosage group and high-dosage group P＜0.05, low-dosage group P＜0.01), the significant improvement in HDL-C (high-dosage group and low-dosage group P＜0.05，medium-dosgage group P＜0.01) and arteriosclerosis factor (AI) (high-dosage group P＜0.05). Conclusion: Polysaccharides from Pholiota adipose play an important role in the reduction of blood lipid and prevention of arteriosclerosis in mice.

Key words: Pholiota adipose, polysaccharides, hypolipidemia, hypolipidemic effect, mice