Abstract:

An analytical method based on gas chromatography (GC) was proposed for the determination of fipronil residue in tea. Samples were extracted with acetontrile and cleaned up on a Florisil (containing graphitized carbon black) column prior to HPLC analysis with μECD detection. The minimum detectable amount of fipronil was 2 × 10-12 g and the minimum detectable concentration of fipronil in tea was 2μg/kg. Average recoveries of 3 replicates for fipronil in a real sample spiked at 5 levels were within the range of 74.0%－92.0%, with a RSD ranging from 3.26% to 10.55%. The accuracy and sensitivity of the developed method both meet the requirements of pesticide residue analysis.

Key words: fipronil, gas chromatography, tea, solid-phase extraction