Immunomagnetic Capture-Fluorescent PCR (IMC-FPCR) for Rapid Detection of Aspergillus flavus in Foods

doi:10.7506/spkx1002-6630-201010063

Abstract

Abstract:

An immunomagnetic bead based technique combined with fluorescent PCR assay was developed for the detection of Aspergillus flavus in foods. Aspergillus flavus in foods could be captured with the immunomagnetic beads coated with goat antiserum against Aspergillus flavus without the requirement for fungal enrichment and rapidly detected by fluorescent PCR assay. The IMC-FPCR assay exhibited a limit of detection of 2 CFU/mL. Two of 12 commercial food samples were detected by the IMC-FPCR assay to contain toxin genes from carcinogenic fungal strains and the detection rate was 16.7% and one Aspergillus flavus strain was detected in peanut and Xiamen ready-to-eat noodles, respectively. This assay is most suitable for the rapid detection of Aspergillus flavus in foods.

Key words: Aspergillus flavus, immunomagnetic beads, fluorescent PCR, rapid detection

CLC Number:

TS207.4

SU Hui-yu1，XIE Rong-zhen2，NIU Jian-jun2. Immunomagnetic Capture-Fluorescent PCR (IMC-FPCR) for Rapid Detection of Aspergillus flavus in Foods[J]. FOOD SCIENCE, 2010, 31(10 ): 287-291.

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Immunomagnetic Capture-Fluorescent PCR (IMC-FPCR) for Rapid Detection of Aspergillus flavus in Foods

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