Abstract:

Objective: To purify pomegranate peel polyphenols with macroporous adsorbent resins and evaluate their antioxidant activity in vitro. Methods: The crude polyphenol extract obtained from pomegranate peel through ultrasound-assisted extraction was separated and purified with 10 kinds of domestic macroporous adsorbent resins, and the adsorption kinetics was investigated. In addition, four classical antioxidant assessment methods were used to evaluate the antioxidant activity of the purified pomegranate peel polyphenols. Results: The content of pomegranate peel polyphenols in the crude extract was increased from 35.05% to 87.81% using macroporous adsorbent resins D160 and XRD-6 and the content of punicalagin was increased from 21.25% to 73.96%. in vitro antioxidant activity of the extract exhibited a dose-effect relationship relevant to the content of polyphenols, especially punicalagin. Conclusion: The application of macroporous adsorbent resins appears to be feasible for the separation and purification of pomegranate peel extract, and punicalagin is a key antioxidant component in pomegranate peel polyphenol mixtures.

Key words: pomegranate peel polyphenol, macroporous adsorbent resins, purification, antioxidation