FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (11): 178-181.doi: 10.7506/spkx1002-6630-201011038

• Bioengineering • Previous Articles     Next Articles

Preliminary Analysis of Bacterial Flora of the Intestinal Tract of Carp

HOU Jin-hui,CHEN Hong-wei,CAO Ze-hong,GAO Zhao-Jian,CAI Kan   

  1. College of Food Engineering (Bioengineering), Xuzhou Institute of Technology, Xuzhou 221008, China
  • Received:2009-09-21 Online:2010-06-01 Published:2010-12-29
  • Contact: HOU Jin-hui E-mail:houjinhui0@126.com

Abstract:

In this study, 62 bacterial strains were isolated from the intestinal tract of healthy Carp, Carassius auratus. Totally 18 Gram-positive and 44 Gram-negative strains were identified through Gram-staining. Cellulase and amylase production analysis verified 26 amylase-producing strains and no cellulase-producing strains in the intestinal tract of carp. Amylase-producing strain was 41.94% among all the isolated strains. Several strains were identified to be Aeromonas, Shewanella and Pseudomonas through 16S rDNA gene sequencing analysis. Pathogencity and amylase activity of strain F2 was analyzed to reveal that this strain was nonpathogenic for carp and its activity reached the peak level at pH 7 and 32 ℃.

Key words: Carassius auratus, intestinal tract, bacteria, amylase, symbiotic strain

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