Abstract:

Phytoene desaturase (Pds) is a key enzyme in the biosynthesis pathway of carotenoid. The target gene pds and the sequence of tomato fruit specific E8 promoter were amplified by PCR and then cloned into pMD1 vector, respectively. PCR, restriction enzyme map, and sequence analysis were used to identify the reconstructed plasmid. The experimental results confirmed the successful construction of a recombinant plasmid carrying pds gene and promoter E8. The recombinant plasmid successfully transformed Atumefaciens LBA4404 and EHA105. These investigations will provide references for further study of pds expression in tomato fruits.

Key words: phytoene desaturase, fruit specific promoter, vector construction