FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (14): 178-181.doi: 10.7506/spkx1002-6630-201014040

• Analysis & Detection • Previous Articles     Next Articles

Determination of Polyphenols in Different Organic Solvent Extracts of Semen Astragali complanati

ZHANG Qing-an1,2，FAN Xue-hui3，ZHANG Zhi-qi2，MA Xue-yan1，CHEN Yu1，WANG Jun1   

  1. 1. College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China ；
    2. School of Chemistry and Materials Science, Shaanxi Normal University, Xi’an 710062, China ；
    3. College of Sport, Shaanxi Normal University, Xi’an 710062, China
  • Received:2009-10-14 Revised:2010-03-18 Online:2010-07-15 Published:2010-12-29
  • Contact: ZHANG Qing-an1 E-mail:qinganzhang@snnu.edu.cn

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Abstract:

In order to provide an optimal Folin-Ciocalteu assay for determining polyphenols in different organic solvent (ethanol aqueous solution, acetone aqueous solution and methanol aqueous solution all at 60% cocentration) extracts of Semen Astragali complanati, some Folin-Ciocalteu assay conditions were optimized. The results indicated that the optimal conditions for the determination of polyphenols by Folin-Ciocalteu were as follows: 10-fold diluted Folin-Ciocalteu reagent amount 1.00 mL, 7.5 g/100 mL Na2CO3 amount 3 mL, reaction temperature 25 ℃, reaction time 120 min, and determination wavelength 647 nm. A good linear relationship was observed between absorbance and gallic acid concentration within the range of 10－100 μg/mL (y = 0.0081x －0.0249, R2 = 0.9996). In addition, accurate contents of polyphenols in methanol extract, ethanol extract and acetone extract of Semen Astragali complanati diluted to a concentration of 1 g/100 mL was determined by this method.

Key words: Semen Astragali complanati, extract, polyphenols, determination

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