Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Enteromorpha prolifera by Response Surface Methodology

doi:10.7506/spkx1002-6630-201016025

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (16): 117-121.doi: 10.7506/spkx1002-6630-201016025

• Processing Technology • Previous Articles Next Articles

Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Enteromorpha prolifera by Response Surface Methodology

GUO Lei1,2，CHEN Yu1

1. Key Laboratory of Marine Biotechnology of Jiangsu Province, Huaihai Institute of Technology, Lianyungang 222005, China；
2. Jiangsu Marine Resources Development Reasearch Institute, Lianyungang 222004, China

Received:2010-01-27 Revised:2010-07-08 Online:2010-08-25 Published:2010-12-29
Contact: GUO Lei1 E-mail:leiguoo@sina.com

Abstract

Abstract:

We report the use of response surface methodology for the optimization of process conditions for the ultrasonicassisted hot water extraction of polysaccharides from Enteromorpha prolifera. The effects of three independent variables (namely, extraction temperature, length of extraction and ratio of water to material) on the yield of polysaccharides from E. prolifera (PEP) were evaluated. Extraction temperature of 80 ℃, length of extraction of 28 min and ratio of water to material of 63:1 (mL/g) were found optimum. Under these optimized conditions, the experimental value of PEP yield was 25.84 mg/g, in good agreement with the predicted value. Therefore, the optimized process is very reliable.

Key words: Enteromorpha prolifera, polysaccharide, ultrasonic-assisted extraction, response surface methodology, technique optimization

CLC Number:

TS201.1

GUO Lei1,2，CHEN Yu1. Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Enteromorpha prolifera by Response Surface Methodology[J]. FOOD SCIENCE, 2010, 31(16): 117-121.

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Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Enteromorpha prolifera by Response Surface Methodology

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