Isolation and in vitro Antioxidant Activity of Active Peptides from Taihe Black-bone Silky Fowl (Gallus gallus domesticus Brisson) Muscle

doi:10.7506/spkx1002-6630-201019015

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (19): 79-83.doi: 10.7506/spkx1002-6630-201019015

• Basic Research • Previous Articles Next Articles

Isolation and in vitro Antioxidant Activity of Active Peptides from Taihe Black-bone Silky Fowl (Gallus gallus domesticus Brisson) Muscle

LIU Jian-hua，TIAN Ying-gang，WANG Yong，ZHU Sheng，WANG Chun-yan，ZHANG Pan，XIE Ming-yong*

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Received:2010-06-19 Revised:2010-09-09 Online:2010-10-15 Published:2010-12-29
Contact: XIE Ming-yong* E-mail:myxie@ncu.edu.cn

Abstract

Abstract:

The Taihe black-bone silky fowl muscle hydrolysate prepared by papain hydrolysis were separated to obtain active peptides by preparative HPLC with 0.01 mol/L phosphate buffer as the mobile phase, and the separated fractions were then pooled and desalted stepwise by 001 × 16 strong acid cation exchange resin and 201 × 7 strong basic anion exchange resin column chromatography. The hydrolysate and its separated fractions were tested for their in vitro antioxidant effects by hydroxyl free radical scavenging array, lipid peroxidation inhibition assay and measuring the ability to chelate Fe2+ and Cu2+ and compared with carnosine and ascorbic acid. Totally 13 fractions were separated from the Taihe black-bone silky fowl muscle hydrolysate, and all of them had obvious antioxidant effect in a concentration-dependent way. Most of the fractions presented stronger abilities to scavenge hydroxyl free radicals, inhibit lipid peroxidation and chelate Fe2+ than the hydrolysate.

Key words: active peptides derived from Taihe black-bone silky fowl muscle, antioxidation, free radical scavenging ability, peroxidation inhibition, metal chelating ability

CLC Number:

R282.74

LIU Jian-hua，TIAN Ying-gang，WANG Yong，ZHU Sheng，WANG Chun-yan，ZHANG Pan，XIE Ming-yong*. Isolation and in vitro Antioxidant Activity of Active Peptides from Taihe Black-bone Silky Fowl (Gallus gallus domesticus Brisson) Muscle[J]. FOOD SCIENCE, 2010, 31(19): 79-83.

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Isolation and in vitro Antioxidant Activity of Active Peptides from Taihe Black-bone Silky Fowl (Gallus gallus domesticus Brisson) Muscle

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