Abstract:

Flavonoid glycosides were prepared from rape bee pollen, and their ability to scavenge DPPH free radicals and reducing power in simulated chemical systems were determined. The antioxidant activity of the extracted flavonoid glycosides was not as strong as that of kaempferol or quercetin. The antioxidant activity of the extracted compounds in vivo against CCl4-induced lipid peroxidation in mice was investigated, and the antioxidant mechanisms were also elucidated. The results showed that the extract could significantly inhibit the generation of MDA in liver, and increase the level of T-AOC in liver and serum. The possible antioxidant mechanism was the generation of flavonoid aglycone with plentiful antioxidant phenolic groups due to the hydrolysis of flavonoid glycosides by in vivo relevant enzymes. Therefore, flavonoid glycosides derived from rape bee pollen had a good protective effect against in vivo oxidative damage.

Key words: rape bee pollen, flavonoid glycosides, antioxidant activity, lipid peroxidation