Abstract:

Black sesame protein was prepared by alkaline extraction and acid precipitation, and then subjected to single enzymatic hydrolysis with neutral proteinase, papain and alkaline protease. Neutral protease was screened out of the three proteases for preparing black sesame protein with higher free radical scavenging activity. Additionaly, the optimization of respective conditions for neutral protease hydrolysis for achieving maximum scavenging rates for hydroxyl, superoxide anion and peroxyl free radicals was carried out using orthogonal array design, and the results showed that the maximum scavenging rates for the above three species of radicals were up to 94.84%, 74.78% and 43.63%, respectively, under optimized hydrolysis conditions.

Key words: sesame protein, antioxidant activity, free radical