Production of 2-Keto-D-Gluconic Acid Using Immobilized Pseudomonas fluorescens AR4

doi:10.7506/spkx1002-6630-201021058

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (21): 258-261.doi: 10.7506/spkx1002-6630-201021058

• Bioengineering • Previous Articles Next Articles

Production of 2-Keto-D-Gluconic Acid Using Immobilized Pseudomonas fluorescens AR4

CHEN Ping1，MIAO Xiao-yan1， ZHANG Xiao-mei1，ZHOU Qiang2，SUN Wen-jing2,3,*

1. Department of Biochemistry, Baoding University, Baoding 071000, China；2. Parchn Sodium Isovitamin C Co. Ltd., Dexing
334221, China；3. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China

Received:2010-08-30 Revised:2010-10-20 Online:2010-11-15 Published:2010-12-29
Contact: SUN Wen-jing E-mail:sunwenjing1919@163.com

Abstract

Abstract:

The cells of Pseudomonas fluorescens AR4 were immobilized by sodium alginate, and the optimization of fermentation conditions for producing 2-keto-D-gluconic acid by immobilized cells were investigated. The results showed that 5.0% of sodium alginate was the optimal carrier for immobilizing Pseudomonas fluorescens AR4 to give a high yield, and the immobilized cells could be reused for 7 times. The optimal concentration of corn steep liquor in fermentation medium was 0.75g/100 mL, and fermentation temperature was between 30 ℃ and 36 ℃. Under the optimal conditions, immobilized cells and free cells could give similar yield and conversion rate of 13.5 g/100 mL and 90.0%, respectively.

Key words: immobilized cell, Pseudomonas fluorescens, 2-keto-D-gluconic acid, sodium alginate, fermentation

CLC Number:

TQ814.2

CHEN Ping1，MIAO Xiao-yan1， ZHANG Xiao-mei1，ZHOU Qiang2，SUN Wen-jing2,3,*. Production of 2-Keto-D-Gluconic Acid Using Immobilized Pseudomonas fluorescens AR4[J]. FOOD SCIENCE, 2010, 31(21): 258-261.

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Production of 2-Keto-D-Gluconic Acid Using Immobilized Pseudomonas fluorescens AR4

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