Abstract:

In order to prepare specific polyclonal antibodies against oviduct glucoprotein (OGP) in Rana Chensinensis Changbaishanensis, the crude fresh OGP-rich extract was purified by Sepharose fast flow anion exchange column and Sephadex G-100 gel column chromatography, and New-Zealand albino rabbits were immunized with the purified OGP for the generation of polyclonal antisera. The purification and identification of the polyclonal antisera obtained were performed using the caprylic acid-saturated ammonium sulfate method. The titer of the purified polyclonal antibodies was measured by enzyme linked immunosorbent assay to be 1:64000, which were characterized by high sensitivity, specificity and titer. Moreover, the polyclonal antibodies prepared in this study were used for the immunohistochemical localization and detection of OGP secreting cells in the oviduct of Rana Chensinensis Changbaishanensis, which was expected to lay an experimental foundation for determining OGP in the culture product of OGP secreting cells.

Key words: Rana chensinensis Changbaishanensis, oviduct glucoprotein, antibody, immunohistochemistry