Optimization of Expression Conditions for Listeria monocytogenes p60 Protein

doi:10.7506/spkx1002-6630-201023037

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 160-163.doi: 10.7506/spkx1002-6630-201023037

• Bioengineering • Previous Articles Next Articles

Optimization of Expression Conditions for Listeria monocytogenes p60 Protein

LU Tian，CAO Zheng-mao，WU Hai-tao，WANG Xiao-hong

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2010-01-25 Revised:2010-11-11 Online:2010-12-15 Published:2010-12-29
Contact: WANG Xiao-hong E-mail:wxh@mail.hzau.edu.cn

Abstract

Abstract:

In this study, the optimal conditions for the expression of Listeria monocytogenes p60 protein, for which the recombinant expression vector pET-28a-p60 and the recombinant E. coli BL21(DE3) host strain were available, were investigated. The results showed that the optimal expression process determined was that the host strain was enriched to a OD600nm value between 0.7 and 0.8, and IPTG was then added to a final concentration of 1 mmol/L, followed by 6 h expression on a shake table with a shaking speed of 150 r/min at 25 ℃, and that after the expression, the content of p60 protein as a percentage of total protein content was 42.32%, and soluble protein occupied around 85.36% of the total p60 protein.

Key words: Listeria monocytogenes, p60 protein, expression condition, optimization

CLC Number:

Q815

LU Tian，CAO Zheng-mao，WU Hai-tao，WANG Xiao-hong. Optimization of Expression Conditions for Listeria monocytogenes p60 Protein[J]. FOOD SCIENCE, 2010, 31(23): 160-163.

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Optimization of Expression Conditions for Listeria monocytogenes p60 Protein

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