Induced Mutagenesis of Mortierella M11for Improving the Abilities to Produce Lipid and γ-Linolenic Acid

doi:10.7506/spkx1002-6630-201023071

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (23): 322-325.doi: 10.7506/spkx1002-6630-201023071

• Bioengineering • Previous Articles Next Articles

Induced Mutagenesis of Mortierella M11for Improving the Abilities to Produce Lipid and γ-Linolenic Acid

ZHANG Chao1,2，XU Zhou2，WEI Qin2，LI Zheng-guo1,*

1. College of Bio-engineering, Chongqing University, Chongqing 400044, China；
2. Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan, College of Life Science and
Food Engineering, Yibin University, Yibin 644000, China

Received:2010-09-03 Revised:2010-11-23 Online:2010-12-15 Published:2010-12-29
Contact: LI Zheng-guo1,* E-mail:zgli6@163.com

Abstract

Abstract:

In order to improve the productivity of lipid and γ-linolenic acid in Mortierella M11, the mutagenesis of Mortierella M11 was induced by UV irradiation, DES treatment and their combination, respectively. All the three mutant strains of Mortierella M11 obtained exhibited higher contents of lipid and γ-linolenic acid in comparison with it, and the combined induction was the most effective mutagenesis approach. The mutant strain M117 obtained through the combined induction of UV irradiation and DES treatment showed 41.3% lipid content, 9.28% GLA content and 1.52 g/L GLA yield, with incremental percentages of 11.26%, 11.81% and 24.59% as compared to the original strain, respectively. After nine generations of passage, the abilities of this stain to produce lipid and γ-linolenic acid were both kept stable.

Key words: Mortierella, UV mutagenesis, DES mutagenesis, combinatorial mutagenesis, lipid, γ-linolenic acid

CLC Number:

TS201.3

ZHANG Chao1,2，XU Zhou2，WEI Qin2，LI Zheng-guo1,*. Induced Mutagenesis of Mortierella M11for Improving the Abilities to Produce Lipid and γ-Linolenic Acid[J]. FOOD SCIENCE, 2010, 31(23): 322-325.

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Induced Mutagenesis of Mortierella M11for Improving the Abilities to Produce Lipid and γ-Linolenic Acid

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