Abstract: The objective of this study was to evaluate the defecating function of Biofidobacterium animal BBMN68 on constipated mice. Male Balb/c mice were randomly divided into control group, model control group, positive control group and 3 dosages groups. Mice of 3 dosages groups were orally administered with different concentrations of live B. BBMN68 (1 × 107, 1 × 108, 1 × 109 CFU/mL) at 0.1 mL/(kg·d) dose once a day, and mice of control group as well as model control group were administered with 10% skim milk liquid 0.1mL/(kg·d), while the mice of positive control group with 1 × 108 CFU/mL B. animal at 0.1 mL/(kg·d). Mice constipation model was established by using compound diphenoxylate. Each group was respectively treated with corresponding drugs, and then the time of defecation of the first black feces, the character of feces within 5 hours, and the promoting rate of ink were measured. Results: the mice administrated with B. BBMN68 for 7 days had the shorter time of defecation of the first black feces, and the number and weight of mice feces within 5 hours were increased; when the mice were administered with BBMN68 stain for 15 days, the ink propelling rates were increased. Conclusion: B. animal strain BBMN68 strain has the potential defecation function in constipation mice.

Key words: bifidobacteria, defecation, mice, constipation