Abstract: Objective: To develop a method for the rapid qualitification of glutin-adulterated milk. Methods: For the precipitation of milk proteins, Hg(NO3)2 solution was used, and the supernatant resulting from centrifugation was then added with saturated picric acid solution. After thorough mixing, whether or not the reaction system becomes turbid was identified by comparison with a negative control. The sensitivity of the established method was evaluated by using simulated adulterated milk samples with varying amounts of glutin. Meanwhile, Hg(NO3)2 solution amount and centrifugation conditions were optimized. Results: Addition of a half volume of 1.4 g/100 mL Hg(NO3)2 solution to milk samples provided optimum precipitation of milk proteins. The detection sensitivity of the method for glutin was 300 mg/100 mL. Conclusion: This method is rapid, convenient and highly sensitive, and has the potential to be developed into reagent kit products, which can meet the requirements for detection of real samples.

Key words: adulterated milk, glutin, reagent kit