FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (5 ): 12-15.doi: 10.7506/spkx1002-6630-201105003

• Basic Research • Previous Articles     Next Articles

Purification of Peanut Allergen Ara h1

WU Xu-li1,2,XIAO Jie2,LIU Zhi-gang2,*,LU Zhi-ping3,LIU Jun-jie2   

  1. 1. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510642, China;
    2. Allergy and Immunology Institute, Shenzhen University, Shenzhen 518060, China;
    3. Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen 518060, China
  • Received:2010-05-13 Revised:2011-01-19 Online:2011-03-15 Published:2011-03-03
  • Contact: LIU Zhi-gang E-mail:lzg@szu.edu.cn

Abstract: In order to obtain the peanut allergen Ara h1, ammonium sulfate precipitation and size exclusion chromatography were used for the purification. The identification of peanut allergens was carried out using SDS-PAGE and Western blot analysis. The results indicated that peanut allergen Ara h1 was isolated with purity of more than 90%.

Key words: peanut, Ara h1, protein, purification, allergy

CLC Number: