Selection of Pre-enrichment Methods for Yersinia enterocolitica

doi:10.7506/spkx1002-6630-201105036

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (5 ): 168-171.doi: 10.7506/spkx1002-6630-201105036

• Bioengineering • Previous Articles Next Articles

Selection of Pre-enrichment Methods for Yersinia enterocolitica

NIU Lei1，ZHU Chang-qing1,2，ZHOU Guang-hong1,*，XU Xing-lian1， LI Bin1，JIANG Yun

1. Nantional Center of Meat Quality and Safety Control, Nanjing Agricultual University, Nanjing 210095, China；
2. Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 200001, China

Received:2010-05-17 Revised:2011-01-18 Online:2011-03-15 Published:2011-03-03
Contact: NIU Lei E-mail:2008108060@njau.edu.cn

Abstract

Abstract: Yersinia enterocolitica has been paid so much attention as a kind of foodborne pathogen. The pre-enrichment of Yersinia enterocolitica was conducted by using the cultivation in TSB, PSB and ITC media. The pre-enrichment effect was evaluated by real-time PCR. The results indicated that the cultivation in TSB medium was better than other two media for pure Yersinia enterocolitica system. The enrichment time was 24 h. In addition, an enhanced sensitivity and shorten enrichment time (8 h) of Yersinia enterocolitica were achieved in the mixed microbe system containing TSB and ITC. Therefore, PSB can be used in the detection of Yersinia Enterocolitica in samples.

Key words: Yersinia enterocolitica, pre-enrichment, real-time PCR, culture medium, foodborne pathogen

CLC Number:

TS251.1

NIU Lei1，ZHU Chang-qing1,2，ZHOU Guang-hong1,*，XU Xing-lian1， LI Bin1，JIANG Yun. Selection of Pre-enrichment Methods for Yersinia enterocolitica[J]. FOOD SCIENCE, 2011, 32(5 ): 168-171.

[1]	YANG Huixuan, LUO Xin, ZHU Lixian, YANG Xiaoyin, HAN Guangxing, DONG Pengcheng, ZHANG Yimin. Progress in Application of Bacteriophages in Control of Meatborne Pathogens [J]. FOOD SCIENCE, 2021, 42(9): 293-301.
[2]	REN Hongrong, LI Miaoyun, ZHU Yaodi, ZHAO Gaiming, ZHAO Lijun, WU Huilin, XIAO Kang, CUI Wenming. Recent Progress in Hazards and Control of Clostridium perfringens in Foods [J]. FOOD SCIENCE, 2021, 42(7): 352-359.
[3]	SUN Tianmei, LIU Yangtai, WANG Xiang, DONG Xiaolu, LIU Hong, LI Hongmei, DONG Qingli. Censored Data Analysis in Estimation of Foodborne Pathogen Contamination: A Review [J]. FOOD SCIENCE, 2021, 42(17): 325-332.
[4]	NING Yawei, HOU Linlin, YU Tongyue, HAN Panpan, FU Yunan, LI Mingrui, WANG Zhixin, JIA Yingmin. Detection and Identification of Phenyllactic Acid in Chinese Vinegar and Its Combined Bacteriostatic Effect with Acetic Acid [J]. FOOD SCIENCE, 2021, 42(12): 233-241.
[5]	FANG Taisong, WU Yufan, SHI Ningxin, CHANG Min, HU Bin, LIU Yangtai, LI Hongmei, DONG Qingli, WANG Xiang. Recent Understanding of the Lag Phase of Foodborne Pathogens: A Review [J]. FOOD SCIENCE, 2021, 42(11): 253-260.
[6]	DU Yan, CHEN Fusheng, CHEN Chen, LIU Ying. Comparative Evaluation of DNA Extraction Methods for Detection of Transgenic Soybean Protein and Analysis of lectin Gene Degradation during Soybean Protein Extraction [J]. FOOD SCIENCE, 2020, 41(4): 102-111.
[7]	CHEN Xin, HU Lingping, YING Yubin, LIU Jialin, HU Yaqin, DING Tian. A Review of Cross-Adaptation and Underlying Molecular Mechanism of Foodborne Pathogens [J]. FOOD SCIENCE, 2020, 41(23): 243-254.
[8]	XIN Liang, ZHANG Lanwei. Recent Progress in Nucleic Acid-Microfluidic Chips Used for Detection of Foodborne Pathogens: A Review [J]. FOOD SCIENCE, 2020, 41(23): 266-272.
[9]	QIU Jianqing, LI Shiyang, YE Qianwen, LIN Shaoling, ZENG Shaoxiao, HU Jiamiao. Recent Progress in Sonodynamic Technology in the Field of Food Sterilization [J]. FOOD SCIENCE, 2020, 41(19): 245-252.
[10]	WU Yufan, WANG Xiang, CUI Siyu, SHAO Jingdong. Prevalence and Detection of Arcobacter as an Emerging Foodborne Pathogen in Foods: A Review [J]. FOOD SCIENCE, 2019, 40(7): 281-288.
[11]	XIONG Suyue, MI Ruifang, CHEN Xi, QI Biao, LI Jinchun, LI Jiapeng, QIAO Xiaoling, WANG Shouwei. Multiplex PCR for Simultaneous Detection of Four Food-Borne Bacteria and Common Mold [J]. FOOD SCIENCE, 2019, 40(4): 305-311.
[12]	CAO Xiao, ZHAO Lichao, CHEN Xun, XIE Hui, ZHANG Jingfeng, LIU Zhuokun, WANG Li. Progress in the Application of Immunomagnetic Separation in Rapid Detection of Foodborne Pathogens [J]. FOOD SCIENCE, 2019, 40(15): 338-345.
[13]	YUE Siyuan, SU Liang, REN Pengcheng, LIU Yangtai, WANG Xiang, LIU Qing, DONG Qingli. Progress in Modeling of Foodborne Pathogen Growth at Lag Phase [J]. FOOD SCIENCE, 2019, 40(1): 313-318.
[14]	LIU Yangtai, SUN Wanxia, LIU Baolin, DONG Qingli. Quantitative Exposure Assessment of Listeria monocytogenes in Raw Pork under Three Transportation Modes [J]. FOOD SCIENCE, 2019, 40(1): 85-91.
[15]	JIANG Ronghua, WANG Wen, CAI Zheng, LIN Yuhai, DONG Qingli. Progress in Foodborne Pathogen Cross-Contamination and Risk Assessment during Meat Processing [J]. FOOD SCIENCE, 2018, 39(7): 305-311.

Selection of Pre-enrichment Methods for Yersinia enterocolitica

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments