Determination of Ferulic Acid in Wheat Bran by Derivatization Combined with Spectrophotometry

doi:10.7506/spkx1002-6630-201106035

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (6 ): 155-158.doi: 10.7506/spkx1002-6630-201106035

• Analysis & Detection • Previous Articles Next Articles

Determination of Ferulic Acid in Wheat Bran by Derivatization Combined with Spectrophotometry

YANG Xue，YAO Yan-yan，ZHANG Zhi-qing*

College of Food Science, Sichuan Agricultural University, Ya’an 625014, China

Received:2010-05-07 Revised:2011-01-07 Online:2011-03-25 Published:2011-03-03
Contact: Zhi-Qing ZHANG E-mail:zqzhang721@163.com

Abstract

Abstract: Objective: To establish a determination method of ferulic acid in wheat bran by derivatization combined with spectrophotometry. Methods: Ferulic acid was analyzed spectrophotometrically at 720 nm. The color-developing agent was the mixture of 0.6 g/100 mL FeCl3 and 0.9 g/100 mL K3Fe(CN)6] (1:0.9). Results: A good linear relationship in the concentration range of 2.36－14.16μg/mL for ferulic acid (r = 0.9996) was observed, and the detection limit of this developed method was 0.022μg/mL. The average recovery rate of this method was 103.1%. Conclusion: This developed method is characteristics of simple operation, accurate results and high average recovery rate, which is suitable for the determination of ferulic acid in wheat bran.

Key words: wheat bran, ferulic acid, derived spectrophotometry

CLC Number:

TS210.9

YANG Xue，YAO Yan-yan，ZHANG Zhi-qing*. Determination of Ferulic Acid in Wheat Bran by Derivatization Combined with Spectrophotometry[J]. FOOD SCIENCE, 2011, 32(6 ): 155-158.

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Determination of Ferulic Acid in Wheat Bran by Derivatization Combined with Spectrophotometry

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