Abstract: An immune-deficient mouse model was established by intraperitoneal injection of cyclophosphamide (CTX) to explore the immune enhancement and antioxidant capability of crude polysaccharides (SH) from Phellinus banmii and purified polysaccharide fractions (P-47000 and P-8700). Meanwhile, the bioactivity differences among SH, P-47000 and P-8700 were investigated. The results showed that SH, P-47000, and P-8700 could enhance immune function of CTX-induced immune-deficient mice to some extent. The order of immune enhancement function from strong to weak was P-8700, SH and P-47000. P-47000 exhibited a lower reducing power than SH and P-8700. However, no significant difference in reducing power between SH and P-8700 was observed. In addition, P-47000 revealed lower ability to scavenge superoxide anion free radicals than SH and P-8700. Moreover, P-8700 revealed stronger hydroxyl free radical scavenging capacity with a scavenging rate of 53.421%, which was obviously higher than that of SH and P-47000. All the polysaccharides had high scavenging capability against DPPH free radicals. SH and P-8700 revealed the highest and lowest scavenging capability on DPPH free radicals with a scavenging rate of respectively 93.221% and 66.435%.

Key words: Phellinus baumii, intracellular polysaccharide, immune activity, antioxidant activity