Optimization of Purification Process for DPPH Free Radical Scavenging Components from Acidic Aqueous Extract from Chinese Date Honey by Macroporous Resin Adsorption

doi:10.7506/spkx1002-6630-201116021

Abstract

Abstract: Solvent extraction and purification by macroporous resin adsorption were used to acquire DPPH free radical scavenging components from Chinese date honey. Extraction solvent type, macroporous resin type, sample loading amount, sample loading flow rate, eluent type, elution flow rate and elution volume were optimized. Acidified ultrapure water (adjusted to pH 2 with HCl) was better solvent for DPPH free radical scavenging components in Chinese date honey than ultrapure water. Among four types of macroporous resin investigated, XDA-2 resin was found to be the most suitable choice. The optimal adsorption and desorption parameters of XDA-2 resin for purifying DPPH free radical scavenging components extracted from Chinese date honey of the same weight were sample loading flow rate of 1.0 mL/min, 80% ethanol aqueous solution as the eluent, elution flow rate of 4.0 mL/min and elution volume of 2 BV.

Key words: Chinese date honey, antioxidant activity, DPPH free radical scavenging components, resin

CLC Number:

S896.1

MU Xue-feng，SUN Li-ping，XU Xiang，PANG Jie，HE Wei，HUANG Lan，SHEN Xin-feng. Optimization of Purification Process for DPPH Free Radical Scavenging Components from Acidic Aqueous Extract from Chinese Date Honey by Macroporous Resin Adsorption[J]. FOOD SCIENCE, 2011, 32(16): 98-102.

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Optimization of Purification Process for DPPH Free Radical Scavenging Components from Acidic Aqueous Extract from Chinese Date Honey by Macroporous Resin Adsorption

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