Purification and Physicochemical Analysis of Polysaccharides from Intestinal Tract of Apostichopus japonicus

doi:10.7506/spkx1002-6630-201117023

Abstract

Abstract: Objective: To purify and physicochemically analyze polysaccharides from the intestinal tract of Apostichopus japonicus. Methods: Two polysaccharides, named as polysaccharides A and B, respectively, were isolated and purified by using SephadexG-100 column from the intestinal tract of Apostichopus japonicus, and polysaccharide A was selected to study its physicochemical properties. Its purity was evaluated by SephadexG-100 column chromatography and agarose gel electrophoresis. The relative molecular mass was determined by Sephadex G-200 column chromatography. Monosaccharide components were analyzed by high performance liquid chromatography (HPLC) and their structures were identifiedby infrared spectroscopy and nuclear magnetic resonance. Results: The purified polysaccharide A was composed of GlcUA, GalN, Fuc, and a small amount of GlcN and Gal. Fucose had two configurations including the forms of α-fucose sulfate and β-fucose sulfate residues. This polysaccharide had a relative molecular mass of approximately 18.8 × 104 D. Conclusion: The purified polysaccharide A is an acidic polysaccharide, suggesting that it belongs to the chondroitin sulfate class.

Key words: intestinal tract polysaccharide, purification, component analysis, structure identification, Apostichopus japonicus

CLC Number:

TS254.1

LIN Wei-wei， ZHANG Jian， WANG Mao-jian，CHANG Yao-guang，WANG Gong-ming. Purification and Physicochemical Analysis of Polysaccharides from Intestinal Tract of Apostichopus japonicus[J]. FOOD SCIENCE, 2011, 32(17): 118-122.

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Purification and Physicochemical Analysis of Polysaccharides from Intestinal Tract of Apostichopus japonicus

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