FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (17): 181-185.doi: 10.7506/spkx1002-6630-201117037

• Basic Research • Previous Articles     Next Articles

Circular Dichroism and Chromatographic Studies of Fish Skin Collagens

ZHANG Jun-jie1,2,DUAN Rui1,2,WU Mei-hua1,YE Chao1,Kunihiko KONNO3   

  1. (1.Jiangsu Key Laboratory of Marine Biotechnology, Lianyungang 222005, China; 2.School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005, China; 3.Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan)
  • Published:2011-08-30

Abstract: Acid soluble collagens (ASC) were isolated from carp (Cyprinus carpio) skin and cod (Gadus morhua) skin. Partial biochemical properties of both ASCs were investigated and compared. Denaturation temperatures, measured by melting point using circular dichroism (CD), was 36 ℃ for carp skin ASC and 19.0 ℃ for cod skin ASC, which were in agreement with the amino acid contents of the two collagens. SP-Toyopearl column chromatographic analysis showed that both skin collagens consisted of three kinds of α chains (α1, α2 and α3), which may exist in (α1)2α2 and α1α2α3 hetero-trimer. The pepsin digestion patterns of α1 chain of carp and cod skin ASCs were considerably different from each other.

Key words: fish skin, collagen, chromatography, circular dichroism

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