Isolation, Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle (Zophobas morio) Larvae

doi:10.7506/spkx1002-6630-201118007

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (18): 30-33.doi: 10.7506/spkx1002-6630-201118007

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Isolation, Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle (Zophobas morio) Larvae

ZHANG Jian-xin，GUO Qian，HE Gui-mei，LIU Na

(College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China)

Received:2018-06-20 Revised:2018-06-20 Online:2011-09-25 Published:2011-09-24

Abstract

Abstract: Objective: To isolate and purify water soluble protein from giant mealworm beetle (Zophobas morio) larvae and evaluate its antioxidant activity. Methods: The water soluble protein in giant mealworm beetle larvae was extracted with pH 7.4 phosphate buffer, purified by ammonium sulfate precipitation, and fractionated by Sephadex G-100 column chromatography to obtain fractions P1, P2 and P3. Fraction P2 was further fractionated by DEAE-Sepharose FF column chromatography into subfractions P2M1, P2M2 and P2M3. The antioxidant activities of each fraction and each subfraction were investigated by DPPH and hydroxyl free radical scavenging assays. Results: Subfraction P2M2 presented the highest DPPH and hydroxyl free radical scavenging rates, which were 99.42% and 55.11%, respectively.

Key words: giant mealworm beetle, protein, isolation and purification, antioxidant activity

CLC Number:

TQ936.2

ZHANG Jian-xin，GUO Qian，HE Gui-mei，LIU Na. Isolation, Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle (Zophobas morio) Larvae[J]. FOOD SCIENCE, 2011, 32(18): 30-33.

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Isolation, Purification and Antioxidant Activity Evaluation of Water Soluble Protein from Giant Mealworm Beetle (Zophobas morio) Larvae

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