FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (18): 185-189.doi: 10.7506/spkx1002-6630-201118039

• Analysis & Detection • Previous Articles     Next Articles

Chromatographic Analysis of Phospholipids in Rape, Chrysanthemum, and Lotus Bee Pollens

HE Wei1,2,XU Xiang1,SUN Li-ping1,*,PANG Jie2,HUANG Lan1,2,MU Xue-feng1,2,SHEN Xin-feng1,2   

  1. (1. Bee Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100093, China; 2. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
  • Online:2011-09-25 Published:2011-09-24

Abstract: The phospholipids in rape, chrysanthemum, and lotus bee pollens were separated and purified by TLC, and were quantitatively determined by HPLC. The results indicated that the total phospholipid contents in three bee pollens were from 1.19 to 3.98 g/100 g with a significant difference (P<0.01). Phosphatidylchuline (PC) was the predominant phospholipid in bee pollen, representing 34.30%-59.69% of total phospholipids. Phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidyl ethanolamine (PE), phosphatidylcholine (PC) and lysophosphatidylcholine (LPC) were detected in rape bee pollen, but no PI in chrysanthemum bee pollen, and neither PI nor LPC in lotus bee pollen. As a conclusion, rape bee pollen contains the most phospholipids with the largest total content among the three sources investigated.

Key words: bee pollen, high-performance liquid chromatography (HPLC), phospholipids, content

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